There had been questions as to whether the aircraft would be able to depart from the much shorter runway at Jabara.
The Dreamlifter normally needs a runway of 2,780m (9,119ft) to get airborne at maximum weight; Jabara's runway is only 1,860m long.
A tug was dispatched to the airport to turn around the giant cargo plane.
Brad Christopher of the Wichita Airport Authority told the Associated Press news agency earlier that the company operating the aircraft had "assured us they've run all the engineering calculation and performance and the aircraft is very safe for a normal departure at its current weight and conditions here".
The Dreamlifter, which landed at Jabara on Wednesday evening, is a modified 747-400 passenger aeroplane, which can carry more cargo by volume than any aeroplane in the world, according to Boeing.
The aerospace company uses its fleet of four Dreamlifters to transport large assembled components of its 787 Dreamliner from suppliers around the world to the final assembly location in Washington state.
